Hepatoprotective and Hepatotheraputic Effects of Propolis against D- Galactosamine/lipopolysaccharide-induced Liver Damage in Rats

Objective: The aim of the present study was to investigate the potential hepatoprotective and hepatotherapeutic activities of propolis against Dgalactosamine and lipopolysaccharide (D-GaIN/LPS)-induced hepatotoxicity in rats. Methods: Hepatotoxicity was induced in rats by intra peritoneal injection of GalN (300 mg/kg) and LPS (30 μg/kg). In the hepatoprotection experiment, propolis was administered orally for 10 days before induction of hepatoxicity. In another experiment (hepatotherapy), propolis was dosed immediately after GalN/LPS injection. Results: Injection of GalN/LPS to rats induced hepatic damage that was manifested by a significant increase in the activities of aminotransferases, alkaline phosphatase, lactate dehydrogenase and levels of tumor necrosis factor-alpha (TNF-α) and total bilirubin in serum. Liver homogenate of intoxicated animals had the lower content of reduced glutathione with increased levels of the hepatic malondialdehyde and caspase-3 enzyme. Histological data presented marked damage in liver sections of intoxicated rats. Oral dosing of propolis before or once immediately after intoxication reversed these altered parameters near to normal values. Conclusion: Liver apoptotic events such as DNA fragmentation and increased caspase-3 activity observed during intoxication were prevented by pre and postpropolis treatment. These results suggest that propolis could afford significant protection and therapy in alleviation of hepatotoxicity.